NBGN-100100 NebuChem™ Protein G Resin.pdf

Size

5ml;10ml;Bulk

Description

NebuChem™ Protein G Resin(CAT#NBGN-100100) is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Average Particle Size

90μm (45-165μm)

Ligand

Recombinant Streptococcal protein G lacking the albumin-binding sites expressed in E.coli. There are 3 IgG binding sites per ligand.

MW of Ligand

~22kDa

PI of Ligand

4.69

Ligand/Resin Ratio

 ~2 mg protein G/ml settled resin

Binding Capacity

 > 20 mg human IgG/ml settled resin

Storage Solution

 1 × PBS containing 20% ethanol

Storage

 2-8 ℃; Freezing is prohibited