NBGN-100101 NebuChem™ Protein A Resin.pdf

Size

5ml;10ml;Bulk

Description

NebuChem™ Protein A Resin(CAT#NBGN-100101) is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Average Particle Size

90μm(45-165μm)

Ligand

Recombinant Streptococcal protein A expressed in E.coli. There are 5 IgG binding sites per ligand.

MW of Ligand

~34kDa

PI of Ligand

5.17

Ligand/Resin Ratio

~2 mg protein A/ml settled resin

Binding Capacity

> 20 mg human IgG/ml settled resin

Matrix

Agarose, 4% 

Storage Solution

20% ethanol 

Storage

2-8 °C; Freezing is prohibited