NBGN-100103 NebuChem™ Enhanced Protein A Resin.pdf

Size

25ml;200ml;1L;Bulk

Description

NebuChem™ Enhanced Protein A Resin(CAT#NBGN-100103) is made of recombinant protein A as ligand, which not only keeps the specific binding capacity to Fc region of immunoglobulin molecules, but also enhances chemical stability under Cleaning-in-place (CIP) conditions. The typical dynamic binding capacity is more than 30 g human IgG/L settled resin. There is no significant decrease of binding capacity after purification/CIP for 200 cycles. In the meanwhile, the NebuChem™ Enhanced Protein A Resin(CAT#NBGN-100103) has a low leakage of ligand (< 15 ng ligand/mg IgG) over a number of purification/CIP cycles. As a high quality antibody purification resin, it is highly suitable for large scale antibody purification, and meets the purification requirements of industry-scale customers.

Composition

Highly cross-linked 4% agarose

Average Particle Size

90μm

Ligand

Alkali-tolerant recombinant protein A (E.coli)

Dynamic binding capacity (DBC)

>30mg human lgG/ ml

Leakage

<15ng/ mg

Recommended flow rate

50 to 300 cm/ h

Chemical stability

Stable in all commonly used buffers

pH working range

ph3~12

CIP stability

0.1-0.5M NaOH