NBGN-100105 NebuChem™ Fast Flow Protein G Resin.pdf

Size

5ml;10ml;Bulk

Description

NebuChem™ Fast Flow Protein G Resin(CAT#NBGN-100105) is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of NebuChem™ Fast Flow Protein G Resin(CAT#NBGN-100105) is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Ligand

Recombinant Streptococcal protein G lacking the albumin-binding sites expressed in E.coli. There are 3 IgG binding sites per ligand.

MW of Ligand

~22kDa

PI of Ligand

4.69

Ligand/Resin Ratio

~2 mg protein G/ml settled resin

Binding Capacity

≥ 20 mg human IgG/ml settled resin

Matrix

4% highly cross-linked agarose

Average Particle Size

90μm (45-165μm)

Recommended flow rate

50-300cm/h

Storage Solution

20% ethanol

Storage

2-8 °C; Freezing is prohibited