NBGN-100106 NebuChem™ Fast Flow Protein A Resin (Column).pdf

Size

1ml;5ml;Bulk

Description

NebuChem™ Fast Flow Protein A Resin (Column)(CAT#NBGN-100106) is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. NebuChem™ Fast Flow Protein A Resin (Column)(CAT#NBGN-100106) can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of NebuChem™ Fast Flow Protein A Resin (Column)(CAT#NBGN-100106) is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc.

Ligand

Recombinant Streptococcal protein A expressed in E.coli. There are 5 IgG binding sites per ligand.

MW of Ligand

~34kDa

PI of Ligand

5.17

Ligand/Resin Ratio

~5 mg protein A / ml settled resin

Binding Capacity

> 40 mg human IgG/ ml settled resin

Matrix

Agarose, 4% highly cross-linked

Average Particle Size

90μm (45-165μm)

Storage Solution

20% ethanol

Storage

2-8 °C; Freezing is prohibited