NBGN-100107  NebuChem™ Fast Flow Protein G Resin (Column).pdf

Size

1ml;5ml;Bulk

Description

NebuChem™ Fast Flow Protein G Resin (Column)(CAT#NBGN-100107) is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of NebuChem™ Fast Flow Protein G Resin (Column)(CAT#NBGN-100107) is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Ligand Recombinant Streptococcal protein G lacking the albumin-binding sites expressed in E.coli. There are 3 IgG binding sites per ligand.

MW of Ligand

~22kDa

PI of Ligand

4.69

Ligand/Resin Ratio

~2 mg protein G/ml settled resin

Binding Capacity

≥ 20 mg human IgG/ml settled resin

Matrix

4% highly cross-linked agarose

Average Particle Size

90μm (45-165μm)

Recommended flow rate

50-300cm/h

Storage Solution

20% ethanol

Storage

2-8 °C; Freezing is prohibited

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. 

Additional adaptors may be needed for other systems. 

The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. 

For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column 

(The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).