NBGN-100109 NebuChem™ Ni-Charged Resin.pdf

Size

10ml;50ml;100ml;Bulk

Description

NebuChem™ Ni-Charged Resin(CAT#NBGN-100109) is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. NebuChem™ Ni-Charged Resin(CAT#NBGN-100109) has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins. 

Matrix

4% cross-linked agarose

Average Particle Size

90μm (45-165μm)

Dynamic binding capacity

≥20 mg of 6xHis-tagged protein (27kDa) /ml settled resin

Storage Solution

1X PBS containing 20% ethanol 

Reagents Compatible with High Affinity Ni-Charged Resin

6 M Gu·HCl

8 M Urea

2% Triton X-100

2% Tween 20

1% CHAPS 

20 mM β-ME

1 mM DTT

4 M MgCl2

5 mM CaCl2

2 M NaCl

50% glycerol

20% ethanol

1 mM EDTA"

Storage

2-8 °C； Freezing is prohibited