NBGN-100111 NebuChem™ Anti-DYKDDDDK Affinity Resin.pdf

Size

1ml;5ml;Bulk

Description

NebuChem™ Anti-DYKDDDDK Affinity Resin(CAT#NBGN-100111) is designed for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK‐tagged protein in the cell lysate can specifically bind to the anti‐DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate. 

Matrix

4% cross‐linked agarose

Ligand

Mouse monoclonal antibody against DYKDDDDK tag, clone G1 

Binding Capacity

~1.5 mg DYKDDDDK‐tagged protein per ml settled resin

Resin Reuse

The resin can be recycled for at least 4 times. 

If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.

Elution Method

Acid, alkaline, neutral, peptide competitive or SDS‐PAGE loading buffer elutions*.

(*If elute with SDS-PAGE loading buffer, the heavy chain of G1 antibody will be denatured and release from the resin.)

Storage

2 - 8°C; Freezing is prohibited