NBGN-100112 NebuChem™ Fast Flow Ni-Charged Resin.pdf

Size

5ml;10ml;Bulk

Description

NebuChem™ Fast Flow Ni-Charged Resin(CAT#NBGN-100112) is a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by three coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. NebuChem™ Fast Flow Ni-Charged Resin(CAT#NBGN-100112) has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. NebuChem™ Fast Flow Ni-Charged Resin(CAT#NBGN-100112) is an excellent choice for high performance purification of polyhistidine-tagged proteins.

Binding capacity

>40 mg of histidine-tagged protein /ml settled resin

Matrix

6% highly cross-linked agarose

Average Particle Size

90μm (45-165μm)

Storage Solution

1X PBS containing 20% ethanol

Storage

2 - 8°C; Freezing is prohibited