NBGN-100113 NebuChem™ Fast Flow Ni-Charged Resin (High Affinity).pdf

Size

5ml;10ml;Bulk

Description

NebuChem™ Fast Flow Ni-Charged Resin (High Affinity)(CAT#NBGN-100113) is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. NebuChem™ Fast Flow Ni-Charged Resin (High Affinity)(CAT#NBGN-100113) has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni Charged Resin FF the excellent choice for high performance purification of polyhistidine-tagged proteins.

Matrix

Spherical 6% highly cross-linked agarose

Average Particle Size

90 µm (45-165 µm)

Binding Capacity

≥50 mg of histidine-tagged protein /mL settled resin

Storage solution

20% ethanol

Storage

2 - 8°C; Freezing is prohibited