NBGN-100114 NebuChem™ Fast Flow Ni-Charged Resin (High Affinity, Column).pdf

Size

1ml;5ml;Bulk

Description

NebuChem™ Fast Flow Ni-Charged Resin (High Affinity, Column)(CAT#NBGN-100114) is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. NebuChem™ Fast Flow Ni-Charged Resin (High Affinity, Column)(CAT#NBGN-100114) has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins. 

Dynamic Binding capacity

>50 mg of histidine-tagged protein /ml settled resin

Matrix

6% highly cross-linked agarose

Average Particle Size

90μm (45-165μm)

Storage Solution

20% ethanol

Storage

stored at 2-8 °C; Freezing is prohibited